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Genomics study of the exposure effect of Gymnodinium catenatum, a paralyzing toxin producer, on Crassostrea gigas defense system and detoxification genes

dc.creatorNORMA GARCIA LAGUNAS
dc.creatorREYNA DE JESUS ROMERO GERALDO
dc.creatorNORMA YOLANDA HERNANDEZ SAAVEDRA
dc.date2013
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/595
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/640
dc.description"Background: Crassostrea gigas accumulates paralytic shellfish toxins (PST) associated with red tide species as Gymnodinium catenatum. Previous studies demonstrated bivalves show variable feeding responses to toxic algae at physiological level; recently, only one study has reported biochemical changes in the transcript level of the genes involved in C. gigas stress response. Principal Findings: We found that 24 h feeding on toxic dinoflagellate cells (acute exposure) induced a significant decrease in clearance rate and expression level changes of the genes involved in antioxidant defense (copper/zinc superoxide dismutase, Cu/Zn-SOD), cell detoxification (glutathione S-transferase, GST and cytochrome P450, CPY450), intermediate immune response activation (lipopolysaccharide and beta glucan binding protein, LGBP), and stress responses (glutamine synthetase, GS) in Pacific oysters compared to the effects with the non-toxic microalga Isochrysis galbana. A sub-chronic exposure feeding on toxic dinoflagellate cells for seven and fourteen days (306103 cells mL21) showed higher gene expression levels. A significant increase was observed in Cu/Zn-SOD, GST, and LGBP at day 7 and a major increase in GS and CPY450 at day 14. We also observed that oysters fed only with G. catenatum (36103 cells mL21) produced a significant increase on the transcription level than in a mixed diet (36103 cells mL21 of G. catenatum+0.756106 cells mL21 I. galbana)in all the analyzed genes. Conclusions: Our results provide gene expression data of PST producer dinoflagellate G. catenatum toxic effects on C. gigas, a commercially important bivalve. Over expressed genes indicate the activation of a potent protective mechanism, whose response depends on both cell concentration and exposure time against these toxic microalgae. Given the importance of dinoflagellate blooms in coastal environments, these results provide a more comprehensive overview of how oysters respond to stress generated by toxic dinoflagellate exposure."
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/dataset/DOI/DOI:10.1371/journal.pone.0072323
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/DOI: 10.1371/journal.pone.0072323
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://journals.plos.org/plosone
dc.relationcitation:García-Lagunas N, Romero-Geraldo R, Hernández-Saavedra NY (2013) Genomics Study of the Exposure Effect of Gymnodinium catenatum, a Paralyzing Toxin Producer, on Crassostrea gigas’ Defense System and Detoxification Genes. Plos One 8(9): e72323. doi:10.1371/journal.pone.0072323
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePlos One
dc.subjectinfo:eu-repo/classification/AUTOR/Oysters, Dinoflagellates, Toxins, Diet, Gene expression, Glutathione chromatography, Bivalves, Detoxification
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/310906
dc.titleGenomics study of the exposure effect of Gymnodinium catenatum, a paralyzing toxin producer, on Crassostrea gigas defense system and detoxification genes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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