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dc.contributor.authorFernández Herrera, Leyberth José
dc.contributor.authorNúñez Vázquez, Erick Julián
dc.contributor.authorHernández Sandoval, Francisco E.
dc.contributor.authorCeseña Ojeda, Daniel Octavio
dc.contributor.authorGarcía Davis, Sara
dc.contributor.authorTeles, Andressa
dc.contributor.authorVirgen Félix, Marte
dc.contributor.authorTovar Ramírez, Dariel
dc.date.issued2024
dc.identifierhttps://cibnor.repositorioinstitucional.mx/jspui/handle/1001/3118
dc.identifier.citation: Fernández-Herrera, L.J.; Núñez-Vázquez, E.J.; HernándezSandoval, F.E.; Ceseña-Ojeda, D.O.; García-Davis, S.; Teles, A.; VirgenFélix, M.; Tovar-Ramírez, D. Morphological, Toxicological, and Biochemical Characterization of Two Species of Gambierdiscus from Bahía de La Paz, Gulf of California. Mar. Drugs 2024, 22, 422.es
dc.identifier.otherhttps://doi.org/ 10.3390/md22090422
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/3341
dc.formatpdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rightsAcceso abiertoes
dc.subjectGambierdiscus, amino acids, pigments, toxicity, ciguatoxins, ciguatera, Gulf of Californiaes
dc.subject.classificationMicrobiologíaes
dc.titleMorphological, Toxicological, and Biochemical Characterization of TwoSpecies of Gambierdiscus from Bahía de La Paz, Gulf of Californiaes
dc.typearticlees
dc.description.abstracten"We describe five new isolates of two Gambierdiscus species from Bahía de La Paz in the southern Gulf of California. Batch cultures of Gambierdiscus were established for morphological characterization using light microscopy (LM) and scanning electron microscopy (SEM). Pigment and amino acid profiles were also analyzed using high-performance liquid chromatography (HPLC-UV and HPLC-DAD). Finally, toxicity (CTX-like and MTX-like activity) was evaluated using the Artemia salina assay (ARTOX), mouse assay (MBA), marine fish assay (MFA), and fluorescent receptor binding assay (fRBA). These strains were identified as Gambierdiscus cf. caribaeus and Gambierdiscus cf. carpenteri. Toxicity for CTX-like and MTX-like activity was confirmed in all evaluated clones. Seven pigments were detected, with chlorophyll a, pyridine, Chl2, and diadinoxanthin being particularly noteworthy. For the first time, a screening of the amino acid profile of Gambierdiscus from the Pacific Ocean was conducted, which showed 14 amino acids for all strains except histidine, which was only present in G. cf. caribeaus. We report the presence of Gambierdiscus and Fukuyoa species in the Mexican Pacific, where ciguatera fish poisoning (CFP) cases have occurred."es


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