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dc.creatorDiana Medina Hernández
dc.creatorMaría Goretty Caamal Chan
dc.creatorMAYELA VARGAS SALINAS
dc.creatorABRAHAM LOERA MURO
dc.creatorAarón Barraza Celis
dc.creatorRAMON JAIME HOLGUIN PEÑA
dc.date2019
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1565
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/2922
dc.description"Background. The begomovirus, squash leaf curl virus (SLCuV) is one of the causal agents of squash leaf curl (SLC) disease, which is among the most destructive diseases of cucurbit crops in tropical, subtropical, and semiarid regions worldwide. This disease was originally reported in the American continent with subsequent spread to the Mediterranean basin. Up to now, SLCuV has only been detected by PCR in Mexico. This study provides the first complete sequence of a Mexican SLCuV isolate from Baja California Sur (BCS). In addition, the genome of the virus was characterized, establishing its phylogenetic relationship with other SLCuV isolates. Methods. The full genome (DNA-A and DNA-B) was amplified by rolling circle amplification, cloned and sequenced and the open reading frames (ORF) were annotated.Virus identification was performed according to the International Committee on Taxonomy of Viruses (ICTV) criteria for begomovirus species demarcation. To infer evolutionary relationship with other SLCuV isolates, phylogenetic and recombination analyses were performed. Results. The SLCuV-[MX-BCS-La Paz-16] genome (DNA-A and DNA-B) had 99% identity with SLCuV reference genomes. The phylogenetic analysis showed that SLCuV- [MX-BCS-La Paz-16] is closely related to SLCuV isolates from the Middle East (Egypt, Israel, Palestine and Lebanon). No evidence of interspecific recombination was determined and iterons were 100% identical in all isolates in the SLCuV clade. Conclusions. SLCuV-[MX-BCS-La Paz-16] showed low genetic variability in its genome, which could be due to a local adaptation process (isolate environment), suggesting that SLCuV isolates from the Middle East could have derived from the southwestern United States of America (USA) and northwestern Mexico."
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.7717/peerj.6774
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2167-8359
dc.relationinfo:eu-repo/semantics/reference/URL/URL: https://peerj.com/articles/6774/
dc.relationcitation:Medina-Hernández D, Caamal-Chan MG, Vargas-Salinas M, Loera-Muro A, Barraza A, Holguín-Peña RJ. 2019. Molecular characterization and phylogenetic analysis of a Squash leaf curl virus isolate from Baja California Sur, Mexico. PeerJ 7:e6774https://doi.org/10.7717/peerj.6774
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePeer J
dc.subjectinfo:eu-repo/classification/AUTOR/Leaf curl disease, Iterons, Recombination, Genetic stability, Squash clade
dc.subjectinfo:eu-repo/classification/cti/6
dc.subjectinfo:eu-repo/classification/cti/31
dc.subjectinfo:eu-repo/classification/cti/3108
dc.subjectinfo:eu-repo/classification/cti/310809
dc.subjectinfo:eu-repo/classification/cti/310809
dc.titleMolecular characterization and phylogenetic analysis of a Squash leaf curl virus isolate from Baja California Sur, Mexico
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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