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Isolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)

dc.creatorMARIA DEL CARMEN FLORES MIRANDA
dc.creatorANTONIO LUNA GONZALEZ
dc.creatorANGEL ISIDRO CAMPA CORDOVA
dc.creatorJESUS ARTURO FIERRO CORONADO
dc.creatorBLANCA OFELIA PARTIDA ARANGURE
dc.creatorHECTOR ABELARDO GONZALEZ OCAMPO
dc.date2012
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1356
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/2706
dc.description"Infectious diseases especially those caused by bacterial and viral pathogens are serious loss factors in shrimp farming. In this study, bacteria were isolated from the gut and hepatopancreas of stressed shrimps obtained from a commercial farm. The isolates were screened on Thiosulfate citrate bile salt sucrose (TCBS) agar plates for the selection of Vibrio species. Presumptive vibrios were characterized through tests for hemolytic and enzymatic activity, hydrophobicity, growth and molecular identification. Three experimental infections were conducted in order to confirm the pathogenicity of selected bacterial strains VHPC18, VHPC23, VHPC24 and VIC30. In the third experimental challenge the LD50 was obtained, it lasted 10 days with 10 shrimp, weighing 6.9 }1.1g, per tank. The treatments in triplicate were: (1) saline solution (control group); (2) 2×105CFU/shrimp; (3) 4×105CFU/shrimp; (4) 2×106CFU/shrimp; (5) 4×106CFU/shrimp, and (6) 8×106CFU/shrimp. In all challenges, water parameters measured during the experimental period remained within optimum ranges. Pathogenicity tests confirmed that the mixture of four vibrio isolates, identified as Vibrio sinaloensis, was virulent for L. vannamei. The LD50 value was 1.178×105CFU/g body weight. V. sinaloensis may act as opportunistic pathogens for cultured L. vannamei."
dc.description"Las enfermedades de etiología infecciosa, especialmente las causadas por patógenos bacterianos y virales ocasionan graves pérdidas en el cultivo de camarón blanco Litopenaeus vannamei. En este estudio se caracterizó: la actividad enzimática y hemolítica; hidrofobicidad; crecimiento e identificación molecular de vibrios aislados del intestino y hepatopáncreas de camarones estresados, obtenidos de una granja comercial, en medio Agar Tiosulfato Citrato Bilis Sacarosa. Además, se realizaron tres infecciones experimentales para confirmar la patogenicidad de las cepas bacterianas seleccionadas VHPC18, VHPC23, VHPC24 y VIC30. En la tercera infección experimental se obtuvo la LD50, el reto duro 10 días, con 10 camarones por tanque con un peso de 6.9 }1.1g. Los tratamientos se realizaron por triplicado: (1) solución salina (grupo control); (2) 2×105UFC/camarón; (3) 4×105UFC/camarón; (4) 2×106UFC/camarón; (5) 4×106UFC/camarón y (6) 8×106UFC/camarón. En todos los retos, los parámetros del agua permanecieron dentro de los intervalos óptimos. Las pruebas de patogenicidad confirmaron que la mezcla de cuatro aislados de Vibrio, identificados como Vibrio sinaloensis, fue virulenta para L. vannamei. El valor de la LD50 fue de 1.178×105UFC/g de peso corporal. Los resultados permiten establecer que las cepas de V. sinaloensis pueden actuar como patógenos oportunistas en L. vannamei cultivado."
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Costa Rica
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 0034-7744
dc.relationinfo:eu-repo/semantics/reference/URL/URL: https://revistas.ucr.ac.cr/index.php/rbt/article/view/3914
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.15517/rbt.v60i2.3914
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceRevista de biología tropical
dc.subjectinfo:eu-repo/classification/AUTOR/Vibrio sinaloensis, vibriosis, hemolytic activity, enzymatic activity, Litopenaeus vannamei
dc.subjectinfo:eu-repo/classification/AUTOR/actividad enzimática, actividad hemolítica
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/240111
dc.subjectinfo:eu-repo/classification/cti/240111
dc.titleIsolation and characterization of infectious Vibrio sinaloensis strains from the Pacific shrimp Litopenaeus vannamei (Decapoda: Penaeidae)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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