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dc.creatorANA RUTH ALVAREZ SANCHEZ
dc.creatorHECTOR GERARDO NOLASCO SORIA
dc.creatorALBERTO PEÑA RODRIGUEZ
dc.creatorCLAUDIO HUMBERTO MEJIA RUIZ
dc.date2018
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/931
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1899
dc.description"Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp."
dc.formatapplication/pdf
dc.languageeng
dc.publisherCentral Fisheries Research Institute, Trabzon, Turkey
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.4194/1303-2712-v18_3_05
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://www.trjfas.org
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 1303-2712
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceTurkish Journal of Fisheries and Aquatic Sciences
dc.subjectinfo:eu-repo/classification/AUTOR/Marine yeast, cell digestibility, cell viability, turbidimetry, reduced sugars
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2403
dc.subjectinfo:eu-repo/classification/cti/230290
dc.subjectinfo:eu-repo/classification/cti/230290
dc.titleIn vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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