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Early Detection of White Spot Syndrome Virus (WSSV) in Isolated Hemocytes of Litopenaeus vannamei

dc.creatorARIADNE HERNANDEZ PEREZ
dc.creatorRossanna Rodríguez Canul
dc.creatorEDGAR TORRES IRINEO
dc.creatorJOSE FERNANDO MENDOZA CANO
dc.creatorDANIEL EDUARDO CORONADO MOLINA
dc.creatorJESUS ALEJANDRO ZAMORA BRISEÑO
dc.creatorJORGE HERNANDEZ LOPEZ
dc.date2017
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/963
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1848
dc.description"To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei ) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 0,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RTPCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min."
dc.formatapplication/pdf
dc.languageeng
dc.publisherScientific Research Publishing, Inc.
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.4236/cellbio.2017.61001
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2325-7792
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://www.scirp.org/journal/cellbio
dc.relationcitation:Hernández-Pérez, A. , Rodríguez-Canul, R. , Torres-Irineo, E. , Mendoza-Cano, F. , Coronado-Molina, D. , Zamora-Briseño, J. and Hernández-López, J. (2017) Early Detection of White Spot Syndrome Virus (WSSV) in Isolated Hemocytes of Litopenaeus vannamei. CellBio, 6, 1-12.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceCellBio
dc.subjectinfo:eu-repo/classification/AUTOR/WSSV, Litopenaeus vannamei , Ie1 Gene, Hemocytes, In Vitro Infection, GLS
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/310911
dc.subjectinfo:eu-repo/classification/cti/310911
dc.titleEarly Detection of White Spot Syndrome Virus (WSSV) in Isolated Hemocytes of Litopenaeus vannamei
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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