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Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

dc.creatorIRASEMA ELIZABETH LUIS VILLASEÑOR
dc.creatorDOMENICO VOLTOLINA LOBINA
dc.creatorBRUNO GOMEZ GIL RODRIGUEZ SALA
dc.creatorFELIPE DE JESUS ASCENCIO VALLE
dc.creatorANGEL ISIDRO CAMPA CORDOVA
dc.creatorJUAN MANUEL AUDELO NARANJO
dc.date2015
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1012
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1844
dc.description"Se estudiaron los perfiles de bandeo de la comunidad bacteriana de juveniles de Litopenaeus vannamei tratados con dos probióticos y expuesto a la bacteria patógena Vibrio parahaemolyticus CAIM 170. Los perfiles de bandeo se construyeron usando el gen 16S rRNA y la técnica PCR-SSCP (Polimorfismo conformacional de cadena sencilla) y los probióticos fueron una mezcla experimental de Bacillus (Bacillus tequilensis YC5-2 y B. endophyticus C2-2 y C3-B) y el probiótico comercial Alibio. El ADN para el análisis PCR-SSCP se obtuvo de los intestinos de camarones tratados durante 20 días con los probióticos, inyectados con 2,5×105 UFC g-1 de V. parahaemolyticus una semana después de la suspensión del tratamiento con probióticos. Camarones no tratados con probióticos sirvieron como control positivo (inyectados con V. parahaemolyticus) y negativo (no inyectados). El análisis de la comunidad bacteriana durante el reto confirmó la presencia del patógeno inyectado en el control positivo y su ausencia en el negativo y en los organismos tratados con probióticos. Durante las 48 h del período experimental se observó una diferencia significativa en la diversidad de la comunidad bacteriana. Los patrones de bandas se agruparon en un grupo a las 0-12 h y en uno diferente después de 48 h y sugirieron que los intestinos de camarón blanco fueron dominados por gamma proteobacteria representados por Vibrio sp. y Photobacterium sp. durante la infección bacteriana. Estos resultados indican que las dos mezclas pueden modular la comunidad bacteriana y pueden ser usadas como probióticos para controlar la vibriosis en camarones juveniles."
dc.description"The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5×105 CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp."
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relationinfo:eu-repo/semantics/datasetURL/URL: //www.lajar.cl/
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/DOI: 10.3856/vol43-issue4-fulltext-15
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 0718-560X
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceLatin American Journal of Aquatic Research
dc.subjectinfo:eu-repo/classification/AUTOR/Litopenaeus vannamei, mezcla de Bacillus, Vibrio parahaemolyticus, comunidad bacteriana, acuicultura.
dc.subjectinfo:eu-repo/classification/AUTOR/Litopenaeus vannamei, Bacillus mix, Vibrio parahaemolyticus, bacterial community, aquaculture.
dc.subjectinfo:eu-repo/classification/cti/1
dc.subjectinfo:eu-repo/classification/cti/25
dc.subjectinfo:eu-repo/classification/cti/2510
dc.subjectinfo:eu-repo/classification/cti/251092
dc.subjectinfo:eu-repo/classification/cti/251092
dc.titleModulación por probióticos de la comunidad bacteriana intestinal de juveniles de Litopenaeus vannamei infectados con Vibrio parahaemolyticus CAIM 170
dc.titleProbiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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