Aislamiento y caracterización de bacterias de la almeja “pata de mula” (Anadara tuberculosa) con potencial probiótico para el cultivo de camarón
Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming
| dc.creator | Ana Claudia Sánchez-Ortiz | |
| dc.creator | ANTONIO LUNA GONZALEZ | |
| dc.creator | ANGEL ISIDRO CAMPA CORDOVA | |
| dc.creator | RUTH ESCAMILLA MONTES | |
| dc.creator | MARIA DEL CARMEN FLORES MIRANDA | |
| dc.creator | JOSE MANUEL MAZON SUASTEGUI | |
| dc.date | 2015 | |
| dc.identifier | http://cibnor.repositorioinstitucional.mx/jspui/handle/1001/1028 | |
| dc.identifier.uri | http://dspace.cibnor.mx:8080/handle/123456789/1840 | |
| dc.description | "En acuicultura, los probióticos han sido utilizados para incrementar respuesta inmune, crecimiento y supervivencia en especies marinas incluyendo camarones y moluscos. Con el propósito de aislar bacterias con un alto potencial probiótico para el cultivo de especies marinas, se obtuvieron homogeneizados del tracto gastrointestinal de adultos de “pata de mula” Anadara tuberculosa para realizar ensayos in vitro e in vivo. Las cepas se evaluaron in vitro para determinar actividad hemolítica, hidrofobicidad, tolerancia a nitrógeno amoniacal, salinidad y pH, cinética de crecimiento, actividad enzimática extracelular, autoagregación y coagregación, y finalmente se identificaron por métodos moleculares. Se seleccionaron siete bacterias; tres con alto grado de hidrofobicidad (>60% de adherencia a p-xileno) y cuatro con hidrofobicidad media, que mostraron diferentes patrones de adhesión a solventes monopolares (cloroformo y acetato de etilo) y alta tolerancia al nitrógeno amoniacal (200 mg L-1 ). Se realizó un ensayo in vivo para evaluar la tasa de crecimiento específico y la respuesta inmune celular en camarón Litopenaeus vannamei, utilizando seis tratamientos: T1 (sin adición de bacterias); T2 (MAt29, Enterococcus casseliflavus); T3 (MAt35, Citrobacter koseri); T4 (GAtB1, Bacillus subtilis subtilis.); T5 (GAt7, Staphylococcus sp.); y T6 (mezcla 1:1:1:1 de T2, T3, T4 y T5). La mejor tasa de crecimiento específico se obtuvo con los tratamientos T6 y T4 adicionados con Bacillus subtilis subtilis. Se encontró una diferencia significativa en el conteo de hemocitos (P < 0,05) para T4 con respecto al grupo control. Las cepas aisladas de A. tuberculosa tuvieron un efecto benéfico sobre el crecimiento y respuesta inmune de L. vannamei, por lo que tienen potencial como probióticos en la acuicultura de organismos marinos." | |
| dc.description | "In aquaculture, probiotics have been tested for enhancing the immune system and promoting growth and survival rate of many marine species like shrimp and mollusks. In order to isolate bacteria with a high probiotic potential for marine shellfish aquaculture, homogenates of the gastrointestinal tract from adult mangrove cockle, Anadara tuberculosa, were obtained to perform in vitro and in vivo assays. Isolates were tested in vitro for hemolytic activity, hydrophobicity, tolerance to ammonia nitrogen, salinity and pH as well as for growth kinetics, extracellular enzymatic activity, autoaggregation, coaggregation and molecular identification. Three bacteria with high degree of hydrophobicity (>60% adherence to p-xylene) and four bacteria with medium hydrophobicity, which showed different patterns of attachment to monopolar solvents (chloroform and ethyl acetate) and a high tolerance to ammonia nitrogen (200 mg L-1 ), were selected. Six different treatments: T1 (without addition of cultured bacteria); T2 (MAt29, Enterococcus casseliflavus); T3 (MAt35, Citrobacter koseri); T4 (GAtB1, Bacillus subtilis subtilis); T5 (GAt7, Staphylococcus sp.); and T6 (1:1:1:1 mix of strains T2, T3, T4 and T5), were used to evaluate the specific growth rate, and cellular immune response of the shrimp Litopenaeus vannamei. The best specific growth rate was observed for T6 and T4 treatments related to Bacillus subtilis subtilis. A significant difference in total hemocytes count (P < 0.05) was found for T4 treatment with respect to control group. Strains isolated from A. tuberculosa had a beneficial effect on the growth and immune response of L. vannamei, so they have potential use as probiotics in aquaculture of marine shellfish." | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.relation | info:eu-repo/semantics/datasetURL/URL: //www.lajar.cl/ | |
| dc.relation | info:eu-repo/semantics/altIdentifier/DOI/DOI: 10.3856/vol43-issue1-fulltext-11 | |
| dc.relation | info:eu-repo/semantics/reference/ISSN/ISSN: 0718-560X | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.source | Latin American Journal of Aquatic Research | |
| dc.subject | info:eu-repo/classification/AUTOR/Anadara tuberculosa, probióticos, bacilos, respuesta inmune, acuicultura. | |
| dc.subject | info:eu-repo/classification/AUTOR/Anadara tuberculosa, probiotics, bacilli, immune response, aquaculture. | |
| dc.subject | info:eu-repo/classification/cti/1 | |
| dc.subject | info:eu-repo/classification/cti/25 | |
| dc.subject | info:eu-repo/classification/cti/2510 | |
| dc.subject | info:eu-repo/classification/cti/251092 | |
| dc.subject | info:eu-repo/classification/cti/251092 | |
| dc.title | Aislamiento y caracterización de bacterias de la almeja “pata de mula” (Anadara tuberculosa) con potencial probiótico para el cultivo de camarón | |
| dc.title | Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion |
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