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ISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING

dc.creatorJuan Arroyo
dc.creatorADRIAN MUNGUIA VEGA
dc.creatorRICARDO RODRIGUEZ ESTRELLA
dc.creatorJordi Bascompte
dc.date2013
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/830
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1608
dc.description"Premise of the study: Microsatellite primers were developed for the parasitic mistletoe Phoradendron californicum to investigate to what extent population genetic structure depends on host tree distribution within a highly fragmented landscape. Methods and Results: Fourteen unlinked polymorphic and four monomorphic nuclear microsatellite markers were developed using a genomic shotgun pyrosequencing method. A total of 187 alleles plus four monomorphic loci alleles were found in 98 individuals sampled in three populations from the Sonoran Desert in the Baja California peninsula (Mexico). Loci averaged 13.3 alleles per locus (range 4–28), and observed and expected heterozygosities within populations varied from 0.167–0.879 and 0.364–0.932, respectively. Conclusions: Levels of polymorphism of the reported markers are adequate for studies of diversity and fragmentation in natural populations of this parasitic plant. Cross-species amplifi cations in P. juniperinum and P. diguetianum only showed four markers that could be useful in P. diguetianum."
dc.formatapplication/pdf
dc.languageeng
dc.publisherBotanical Society of America
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI:10.3732/apps.1300048
dc.relationinfo:eu-repo/semantics/reference/URL/URL: https://cms.botany.org/home/publications/apps.html
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2168-0450
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceApplications in Plant Sciences
dc.subjectinfo:eu-repo/classification/AUTOR/mistletoe, parasitic plant, Phoradendron californicum, shotgun sequencing, SSR, toji
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2417
dc.subjectinfo:eu-repo/classification/cti/241714
dc.titleISOLATION OF 18 MICROSATELLITE LOCI IN THE DESERT MISTLETOE PHORADENDRON CALIFORNICUM (SANTALACEAE) VIA 454 PYROSEQUENCING
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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