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DEVELOPMENT AND CHARACTERIZATION OF 10 MICROSATELLITE LOCI IN THE GIANT CARDON CACTUS, PACHYCEREUS PRINGLEI (CACTACEAE)

dc.creatorCARINA GUTIERREZ FLORES
dc.creatorOSCAR ADRIAN LOZANO GARZA
dc.creatorJOSE LUIS LEON DE LA LUZ
dc.creatorFRANCISCO JAVIER GARCIA DE LEON
dc.date2014
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/814
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1586
dc.description"Premise of the study: Microsatellite primers were developed for the cardon, a giant columnar cactus ( Pachycereus pringlei ) of the Sonoran Desert, to investigate intraspecifi c genetic patterns of diversity and population structure. Methods and Results: Using 454 GS-FLX technology and bioinformatics tools, microsatellite primers were successfully identified on 282 reads, including di-, tri-, tetra-, penta-, and hexanucleotides. A set of 10 primers were characterized on 80 individuals collected in two areas of the Baja California peninsula, Mexico. All 10 loci were polymorphic, with a mean of 6.3 alleles per locus and overall levels of observed and expected heterozygosities ranging from 0.41 to 0.59 and from 0.40 to 0.57, respectively. Alleles per individual plant ranged from one to four, suggesting a polyploidal genome. Conclusions: These loci should be useful for future investigations of population structure, genetic diversity, and gene fl ow in the cardon cactus."
dc.formatapplication/pdf
dc.languageeng
dc.publisherBotanical Society of America
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI:10.3732/apps.1300066
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2168-0450
dc.relationinfo:eu-repo/semantics/reference/URL/URL: https://cms.botany.org/home/publications/apps.html
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceApplications in Plant Sciences
dc.subjectinfo:eu-repo/classification/AUTOR/Cactaceae, microsatellites, Pachycereus pringlei, pyrosequencing
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2417
dc.subjectinfo:eu-repo/classification/cti/241713
dc.titleDEVELOPMENT AND CHARACTERIZATION OF 10 MICROSATELLITE LOCI IN THE GIANT CARDON CACTUS, PACHYCEREUS PRINGLEI (CACTACEAE)
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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