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Physiological and immune response of Litopenaeus vannamei undergoing the acute phase of the necrotizing hepatopancreatitis disease and after being treated with oxytetracycline and FF

dc.creatorLUIS RAFAEL MARTINEZ CORDOVA
dc.creatorTERESA GOLLAS GALVAN
dc.creatorESTEFANIA GARIBAY VALDEZ
dc.creatorROCIO ARACELY VALENZUELA GUTIERREZ
dc.creatorMARCEL MARTINEZ PORCHAS
dc.creatorMARCO A. PORCHAS CONEJO
dc.creatorArturo Sánchez-Paz
dc.creatorJOSE FERNANDO MENDOZA CANO
dc.date2016
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/789
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1536
dc.description"Se evaluaron respuestas fisiológicas e inmunes de camarones adultos (Litopenaeus vannamei) durante la fase aguda de la enfermedad de la necrosis hepatopancreática, y la eficacia de oxitetraciclina (OXI) y florfenicol (FF) para eliminar el patógeno causante de la enfermedad. Se consideraron cuatro grupos de camarones: tres grupos infectados con NHP-B (tratado con OXI, con FF y un control positivo) y un grupo no infectado (control negativo). Se monitoreó la concentración hemolinfática de glucosa, lactato, acilglicéridos, colesterol, proteína total, aminotransferasas, superóxido dismutasa (SOD), y la expresión de varios genes relacionados con el sistema inmune durante la etapa aguda de la enfermedad y 15 y 20 días después de la administración de antibióticos (dda). Los camarones del control positivo registraron una mortalidad de 100%. La mayoría de los parámetros evaluados se incrementó en camarones infectados antes del uso de antibióticos. Al día 15 dda, lactato y SOD siguieron registrando altos niveles en ambos camarones tratados, mientras que se detectaron altos niveles de transcripción de proteína de coagulación, proteína unidora de lipopolisacáricos, serin proteasa, peroxidasa, lisozima y proteínas del choque térmico 60 y 70, en camarones tratados con OXI. Al día 20 dda NHP-B se siguió detectando en camarones tratados con FF, pero no en los tratados con OXI. Se sugiere que aunque ninguno de los antibióticos per se eliminó a la bacteria, ambos tuvieron un efecto negativo sobre su virulencia. Además, es posible que OXI haya tenido un mejor efecto, permitiendo que los camarones integraran una mejor respuesta inmune a los 15 dda. "
dc.description"The physiological and immune responses of adult shrimp (Litopenaeus vannamei) undergoing the acute phase of the necrotizing hepatopancreatitis (NHP) disease and the efficiency of oxytetracycline (OXI) and florfenicol (FF) to eliminate the pathogen were evaluated. Four shrimp groups were considered: three groups infected with necrotizing hepatopancreatitis bacteria (NHP-B) (two treated with antibiotics and a positive control) and one group non-infected (negative control). Hemolymph concentration of glucose, lactate, acylglycerides, cholesterol, total protein, aminotransferases, superoxide dismutase, and the transcriptional expression of several immune related genes were monitored at the acute phase of the disease, and at 15 and 20 days after administration of antibiotics (daa). Shrimp from the positive control registered a mortality of 100%. NHP-B infection affected the immu-nophysiological response of shrimp; herein, most of the parameters were significantly up regulated in infected shrimp before the use of antibiotics, compared to the negative control. Increased transcriptional levels of clotting protein, lipopolysaccharide and β-1-3-glucan binding protein (LGBP), serine protease, peroxinectin, lysozyme, heat shock proteins (HSP) 60 and 70 were detected in shrimp treated with OXI. At 20 daa NHP-B was still detected in FF-treated shrimp, but not in OXI-treated shrimp. It is hypothesized that despite none of the antibiotics per se eliminated the bacterium, both had a negative effect on its virulence. OXI seems to have a greater effect, allowing shrimp to integrate a better immune response at 15 daa."
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relationinfo:eu-repo/semantics/dataset/URL/URL: //www.lajar.cl/
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/DOI: 10.3856/vol44-issue3-fulltext-12
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 0718-560X
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceLatin American Journal of Aquatic Research
dc.subjectinfo:eu-repo/classification/AUTOR/Litopenaeus vannamei, enfermedad del camarón, necrosis hepatopancreática, sistema inmune, Rickettsia, patógeno intracelular.
dc.subjectinfo:eu-repo/classification/AUTOR/Litopenaeus vannamei, gene expression, shrimp disease, necrotizing hepatopancreatitis, immune system, Rickettsia, intracellular pathogen, necrosis.
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/240111
dc.titleRespuesta fisiológica e inmune de Litopenaeus vannamei durante la fase aguda de la enfermedad de la necrosis hepatopancreática y posteriormente tratado con oxitetraciclina y FF
dc.titlePhysiological and immune response of Litopenaeus vannamei undergoing the acute phase of the necrotizing hepatopancreatitis disease and after being treated with oxytetracycline and FF
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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