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Genetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species

dc.creatorCRISTINA GONZALEZ RUBIO SANVICENTE
dc.creatorRICARDO RODRIGUEZ ESTRELLA
dc.creatorOSCAR ADRIAN LOZANO GARZA
dc.creatorFRANCISCO JAVIER GARCIA DE LEON
dc.date2016
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/784
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1529
dc.description"A set of 16 microsatellite loci was developed and characterized for the Xantus’ hummingbird (Hylocharis xantusii) by using 454 next-generation sequencing. Twenty-five H. xantusii samples from one population were genotyped; all loci were polymorphic, with the number of alleles ranging from three to ten. The mean observed heterozygosity was 0.681 for all loci. No significant linkage disequilibrium was detected, but five loci (Hxan05, 06, 09, 13 and 14) showed deviation from Hardy-Weinberg equilibrium. These microsatellite loci are the first to be characterized for H. xantusii. A moderate to high level of cross-species amplification was observed across the six hummingbird species (31% - 87.5%), with the best cross amplification results observed in the closest related species (H. leucotis, Cynanthus latirostris, Calypte costae). The availability of these molecular tools allows assessing questions integrating population genetics, ecology, conservation, and evolutionary history for H. xantusii and for other phyogenetically related species."
dc.formatapplication/pdf
dc.languageeng
dc.publisherScientific Research Publishing, Inc.
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI:10.4236/ojgen.2016.61003
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2162-4461
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://www.scirp.org/journal/ojgen
dc.relationcitation:González-Rubio, C., Rodríguez-Estrella, R., Lozano-Garza, O.A. and García-De-León, F.J. (2016) Genetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species. Open Journal of Genetics, 6, 19-27.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceOpen Journal of Genetics
dc.subjectinfo:eu-repo/classification/AUTOR/Baja California Peninsula, Hylocharis xantusii, Microsatellites, Pyrosequencing, Cross-Species Amplification
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/240108
dc.titleGenetic Diversity of the Endemic Xantus’ Hummingbird Using 16 Novel Polymorphic Microsatellite Loci, and Their Cross Amplification between Six Related Species
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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