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Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes 1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids

dc.creatorIRENE DE LOS ANGELES BARRIGA SOSA
dc.creatorROSA MARIA GARCIA MARTINEZ
dc.creatorJHOANA DIAZ LARREA
dc.creatorOSCAR ADRIAN LOZANO GARZA
dc.creatorFRANCISCO JAVIER GARCIA DE LEON
dc.date2014
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/785
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1527
dc.description"The endemic silverside fish C. humboldtianum is of great ichtyologycal, economical and cultural relevance in central Mexico and it has been suggested that it is among a group of other “peces blancos”, the most ancestral species. Here we characterized a set of 26 microsatellite loci from the species in order to further assess population and phylogeographic issues that aid in evaluating their highly impacted populations. From 58 primer pairs tested on specimens from Villa Victoria dam (Rio Balsas Basin), 26 loci positively amplified on the species and cross-primed on specimens of the closely related and threatened Poblana alchichica, C. promelas and C. riojai. Loci resolved medium and high genetic variation (mean NA = 14.3, SD = 9.38; mean HO & HE = 0.47, SD = 0.32 and 0.58, SD = 0.32, respectively) and it is expected that these polymorphic loci are also useful in studing threatened atherinopsid species. "
dc.formatapplication/pdf
dc.languageeng
dc.publisherScientific Research Publishing, Inc.
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.4236/ojgen.2014.45032
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 2162-4461
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://www.scirp.org/journal/ojgen
dc.relationcitation:Angeles, B. , García-Martínez, R. , Díaz-Larrea, J. , Garza, O. and García-De-León, F. (2014) Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids. Open Journal of Genetics, 4, 355-361.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceOpen Journal of Genetics
dc.subjectinfo:eu-repo/classification/AUTOR/Endemic, Polymorphism, Villa Victoria, Poblana alchichica, C. promelas, C. riojai
dc.subjectinfo:eu-repo/classification/cti/2
dc.subjectinfo:eu-repo/classification/cti/24
dc.subjectinfo:eu-repo/classification/cti/2401
dc.subjectinfo:eu-repo/classification/cti/240108
dc.titleIsolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes 1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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