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Isolation and risk assessment of Geotrichum spp. in the white shrimp (Litopenaeus vannamei Boone, 1931) from culture ponds

dc.creatorJOSE LUIS OCHOA OCHOA
dc.creatorNORMA ANGELICA OCHOA ALVAREZ
dc.creatorMARIA ANTONIA GUZMAN MURILLO
dc.creatorSERGIO HERNANDEZ VAZQUEZ
dc.creatorFELIPE DE JESUS ASCENCIO VALLE
dc.date2015
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/777
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1525
dc.description"El presente trabajo se realizó con el fin de identificar hongos que invaden algunos de los estanques supralitorales utilizados para el cultivo del camarón en la instalación del CIBNOR, en La Paz, BCS, México durante la temporada de verano. De las paredes y el fondo de los estanques se aislaron e identificaron morfológicamente dos cepas de Geotrichum spp. Se analizó la adhesividad de hongos hacia cultivos primarios de diversos tejidos (hemocitos, branquias, tegumento, e intestino) de camarón blanco (Litopeneaus vannamei) para determinar la infectividad. La actividad de lipasas, amilasas, y proteasa extracelular, fueron evaluadas como factores de virulencia. También se evaluó la supervivencia de post-larvas (PL8) de camarones expuestos a los sobrenadantes del cultivo o filamentos de hongos. Los resultados muestran que las células de tegumento y hemocitos de camarón son susceptibles a la invasión por Geotrichum spp. y que este hongo provoca gran mortalidad de post-larvas de camarón. Por lo tanto, Geotrichum spp. puede ser considerado un patógeno oportunista que podría representar un riesgo grave para la salud de los camarones en cultivo."
dc.description"The present study was done in order to identify the fungus invading some of the supralittoral ponds used for shrimp aquaculture in the CIBNOR facilities in La Paz, Baja California Sur (BCS), México during the summer season. From the walls and bottoms of the ponds, two strains of Geotrichum spp. were isolated and morphologically identified. Fungal adhesion towards hemocytes and primary cultures of various white shrimp (Litopeneaus vannamei) tissues (gill, tegument, and gut) was analyzed to determine infectivity. Extracellular protease, lipase, and amylase activity were evaluated as virulence factors. Survival of shrimp postlarvae (PL8) exposed to fungal culture supernatant or to their filaments was also investigated. The results showed that shrimp tegument cells and hemocytes were very susceptible to Geotrichum spp. invasion, and that this fungus provokes great mortality of post-larvae. Hence, Geotrichum spp. could be considered an opportunistic pathogen that might represent a serious health risk to shrimp in culture."
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relationinfo:eu-repo/semantics/dataset/URL/URL: //www.lajar.cl/
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/DOI: 10.3856/vol43-issue4-fulltext-14
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 0718-560X
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceLatin American Journal of Aquatic Research
dc.subjectinfo:eu-repo/classification/AUTOR/Geotrichum spp., Fusarium solani, Litopenaeus vannamei, micotoxinas, enzimas extracelulares, acuicultura.
dc.subjectinfo:eu-repo/classification/AUTOR/Geotrichum spp., Fusarium solani, Litopenaeus vannamei, mycotoxins, extracellular enzymes, aquaculture.
dc.subjectinfo:eu-repo/classification/cti/1
dc.subjectinfo:eu-repo/classification/cti/25
dc.subjectinfo:eu-repo/classification/cti/2510
dc.subjectinfo:eu-repo/classification/cti/251092
dc.titleAislamiento y evaluación de riesgos de Geotrichum spp. en el camarón blanco (Litopenaeus vannamei Boone, 1931) en estanques de cultivo
dc.titleIsolation and risk assessment of Geotrichum spp. in the white shrimp (Litopenaeus vannamei Boone, 1931) from culture ponds
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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