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Effects and Effectiveness of Two RNAi Constructs for Resistance to Pepper golden mosaic virus in Nicotiana benthamiana Plants

dc.creatorDIANA MEDINA HERNANDEZ
dc.creatorRAFAEL FRANCISCO RIVERA BUSTAMANTE
dc.creatorfrancisco tenllado peralo
dc.creatorRAMON JAIME HOLGUIN PEÑA
dc.date2013
dc.identifierhttp://cibnor.repositorioinstitucional.mx/jspui/handle/1001/750
dc.identifier.urihttp://dspace.cibnor.mx:8080/handle/123456789/1475
dc.description"ToChLPV and PepGMV are Begomoviruses that have adapted to a wide host range and are able to cause major diseases in agronomic crops. We analyzed the efficacy of induced resistance to PepGMV in Nicotiana benthamiana plants with two constructs: one construct with homologous sequences derived from PepGMV, and the other construct with heterologous sequences derived from ToChLPV. Plants protected with the heterologous construct showed an efficacy to decrease the severity of symptoms of 45%, while plants protected with the homologous construct showed an efficacy of 80%. Plants protected with the heterologous construct showed a reduction of incidence of 42.86%, while the reduction of incidence in plants protected with the homologous construct was 57.15%. The efficacy to decrease viral load was 95.6% in plants protected with the heterologous construct, and 99.56% in plants protected with the homologous construct. We found, in both constructs, up-regulated key components of the RNAi pathway. This demonstrates that the efficacy of the constructs was due to the activation of the gene silencing mechanism, and is reflected in the decrease of viral genome copies, as well as in recovery phenotype. We present evidence that both constructs are functional and can efficiently induce transient resistance against PepGMV infections. This observation guarantees a further exploration as a strategy to control complex Begomovirus diseases in the field."
dc.formatapplication/pdf
dc.languageeng
dc.publisherMultidisciplinary Digital Publishing Institute
dc.relationinfo:eu-repo/semantics/reference/ISSN/ISSN: 1999-4915
dc.relationinfo:eu-repo/semantics/reference/URL/URL: http://www.mdpi.com/journal/viruses
dc.relationinfo:eu-repo/semantics/reference/DOI/DOI: 10.3390/v5122931
dc.relationcitation:Medina-Hernández, D.; Rivera-Bustamante, R.F.; Tenllado, F.; Holguín-Peña, R.J. Effects and Effectiveness of Two RNAi Constructs for Resistance to Pepper golden mosaic virus in Nicotiana benthamiana Plants. Viruses 2013, 5, 2931-2945.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceViruses
dc.subjectinfo:eu-repo/classification/AUTOR/Hairpin RNA, Mixed infections, PepGMV, RNAi constructs, ToChLPV, Viral load
dc.subjectinfo:eu-repo/classification/cti/6
dc.subjectinfo:eu-repo/classification/cti/31
dc.subjectinfo:eu-repo/classification/cti/3108
dc.subjectinfo:eu-repo/classification/cti/310809
dc.titleEffects and Effectiveness of Two RNAi Constructs for Resistance to Pepper golden mosaic virus in Nicotiana benthamiana Plants
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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